Fertility is a major health concern with societal implications for birthing couples, especially in tropical areas where adoption rates are still low. As a food tenderizer, flavoring agent, food preservative, and drawing agent in a variety of regional soups, trona has been well accepted. Thus, this study evaluated the effect of trona (sodium sesquicarbonate) on hematology, testosterone, and testicular morphology of sexually mature male rabbits (Oryctolagus cuniculus) during sub-chronic administration.